THE DIFFUSION OF TOBACCO MOSAIC VIRUS PROTEIN IN AQUEOUS SOLUTION

The Partial Reactivation of Formolized Tobacco Mosaic Virus Protein

A marked reactivation of tobacco mosaic virus protein that has been partially or completely inactivated by formaldehyde was obtained by dialysis at pH 3. The activity of partially inactivated virus proteins was generally increased about 10-fold by the reactivation process. It was also found possible to reactivate completely inactive preparations to an appreciable extent. It was shown that the i...

The Viscosity of Tobacco Mosaic Virus Protein Solutions

It is well known that the viscosity of a liquid is a measure of its resistance to shear. Upon the introduction of small solid objects into a viscous medium, the internal motion of the liquid when caused to flow is made more complicated than it would be otherwise and the resistance to shear is thereby increased. Einstein (4) has derived the following formula relating the viscosity of a suspensio...

Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein.

The tobacco mosaic virus (TMV) 30K movement protein (MP) gene was inserted into a full-length cDNA clone of barley stripe mosaic virus (BSMV) RNA beta replacing the triple gene block (TGB). The resulting recombinant ND-MPT genome, consisting of infectious wt transcripts of BSMV RNAs alpha and gamma, together with the hybrid RNA beta transcript, was inoculated onto test plants to study the funct...

Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking

Dimerization of recombinant tobacco mosaic virus movement protein.

The p30 movement protein (MP) is essential for cell-to-cell spread of tobacco mosaic virus in planta. We used anion-exchange chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to obtain highly purified 30-kDa MP, which migrated as a single band in native PAGE. Analytical ultracentrifugation suggested that the protein was monodisperse and dimeric ...